|Architectural Laboratory Water Treatment Marine Air Handling|
(ii) Specimen preparation. Enamel specimens (3 mm in diameter)
were drilled from extracted, sound, human, lower permanent incisors
which had been obtained from oral surgeons and sterilized by soaking
in 3% buffered (neutral) formalin since the time of extraction.
Each specimen was mounted on a polyacrylic rod by using methyl
methacrylate resin. The specimens were first ground by using 600-grade
silicon carbide paper to remove approximately 50 ?m of the surface
and then polished to a high luster with Gamma Alumina (0.05 ?m)
by standard methods. The specimens were then randomly assigned
to test groups, with each group initially composed of 14 specimens.
All specimens were sterilized with ethylene oxide gas. Two specimens
from each group were randomly chosen before treatment and examined
to obtain baseline confocal microscopy data. The 12 specimens
that remained in each group were secured in caries-forming vessels
by gluing the ends of their plexiglass rods to a round plexiglass
base that fit in the bottom of the vessels.
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